Article
Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability.
Molecular cell - 21 Jul 2016
Goldenzweig Adi, Goldsmith Moshe, Hill Shannon E, Gertman Or, Laurino Paola, Ashani Yacov, Dym Orly, Unger Tamar, Albeck Shira, Prilusky Jaime, Lieberman Raquel L, Aharoni Amir, Silman Israel, Sussman Joel L, Tawfik Dan S, Fleishman Sarel J
Abstract excerpt
Upon heterologous overexpression, many proteins misfold or aggregate, thus resulting in low functional yields. Human acetylcholinesterase (hAChE), an enzyme mediating synaptic transmission, is a typical case of a human protein that necessitates mammalian systems to obtain functional expression. We developed a computational strategy and designed an AChE variant bearing 51 mutations that improved core packing,...
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