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Resistance-based directed evolution of nanobodies for higher affinity in prokaryotes.

Biochimica et biophysica acta. General subjects - 1 Nov 2024

Hu Yue, Huo Li, Chen Weiwei, Shen Jinhua, Wang Wenyi

Abstract excerpt

A prokaryotic resistance-based directed evolution system leveraging protein-fragment complementation assay (PCA) was devised, and its proficiency in detecting protein-protein interactions and discriminating varying degrees of binding affinity was demonstrated by two well-characterized protein pairs. Furthermore, we constructed a random mutant library based on the GBPR36K/E45K mutant, characterized by almost no...

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PMID: 39245149
DOI: 10.1016/j.bbagen.2024.130710

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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