Article
Conceptual and methodological advances in cell-free directed evolution.
Current opinion in structural biology - 1 Aug 2015
Dodevski Igor, Markou George C, Sarkar Casim A
Abstract excerpt
Although cell-free directed evolution methods have been used to engineer proteins for nearly two decades, selections on more complex phenotypes have largely remained in the domain of cell-based engineering approaches. Here, we review recent conceptual advances that now enable in vitro display of...
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