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Article

Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations.

Proceedings of the National Academy of Sciences of the United States of America - 19 Jun 2012

Schlinkmann Karola M, Honegger Annemarie, Türeci Esin, Robison Keith E, Lipovšek Daša, Plückthun Andreas

Abstract excerpt

The structural features determining efficient biosynthesis, stability in the membrane and, after solubilization, in detergents are not well understood for integral membrane proteins such as G protein-coupled receptors (GPCRs). Starting from the rat neurotensin receptor 1, a class A GPCR, we generated a separate library comprising all 64 codons for each amino acid position. By combining a previously developed...

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Identifiers

PMID: 22665811
DOI: 10.1073/pnas.1202107109
PMCID: PMC3382542

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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