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Article

Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution.

Journal of molecular biology - 21 Sept 2012

Schlinkmann Karola M, Hillenbrand Matthias, Rittner Alexander, Künz Madeleine, Strohner Ralf, Plückthun Andreas

Abstract excerpt

To identify structural features in a G-protein-coupled receptor (GPCR) crucial for biosynthesis, stability in the membrane and stability in detergent micelles, we developed an evolutionary approach using expression in the inner membrane of Escherichia coli. From the analysis of 800,000 sequences of the rat neurotensin receptor 1, in which every amino acid had been varied to all 64 codons, we uncovered several...

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PMID: 22683350
DOI: 10.1016/j.jmb.2012.05.039

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