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Article

Optimizing the Expression of Human Dopamine Receptors in Escherichia coli.

International journal of molecular sciences - 11 Aug 2021

Boritzki Vanessa, Hübner Harald, Allikalt Anni, Gmeiner Peter, Wöhrl Birgitta M

Abstract excerpt

The human dopamine receptors D2S and D3 belong to the group of G protein-coupled receptors (GPCRs) and are important drug targets. Structural analyses and development of new receptor subtype specific drugs have been impeded by low expression yields or receptor instability. Fusing the T4 lysozyme into the intracellular loop 3 improves crystallization but complicates conformational studies. To circumvent these...

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Source

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Identifiers

PMID: 34445358
DOI: 10.3390/ijms22168647
PMCID: PMC8395450

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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