Article
In vitro selection of zinc finger DNA-binding proteins through ribosome display.
Biochemical and biophysical research communications - 7 Jul 2006
Ihara Hiroshi, Mie Masayasu, Funabashi Hisakage, Takahashi Fumio, Sawasaki Tatsuya, Endo Yaeta, Kobatake Eiry
Abstract excerpt
DNA-binding proteins with sequence specificities have a variety of applications. To create novel functional DNA-binding proteins, in vivo selection methods have been developed. There are, however, crucial problems with such methods, e.g., limitation of library size and difficulty of expression of toxic proteins for the host cells. In order to overcome these problems, we developed a novel way to select DNA-binding...
Topics
- Animals
- Biotinylation
- Cell Line, Tumor
- DNA Primers
- DNA-Binding Proteins
- Early Growth Response Protein 1
- Gene Library
- Genetic Techniques
- In Vitro Techniques
- Mice
- Mutation
- Plasmids
- RNA, Messenger
- Ribosomes
- Zinc Fingers