Article
Virulence phenotype and genetic characteristics of the T32-ISTRATI Shigella flexneri 2a vaccine strain.
Vaccine - 1 May 1991
Venkatesan M, Fernandez-Prada C, Buysse J M, Formal S B, Hale T L
Abstract excerpt
The T32-ISTRATI strain, which has been used as an oral attenuated Shigella flexneri 2a vaccine, has lost the invasive phenotype due to a spontaneous deletion in the shigella virulence plasmid. This deletion has eliminated three plasmid loci (ipaBCDA, invA and virG) that are necessary for production of a positive Sereny test by Shigella species. Virulence in the Sereny test was reconstituted in the T32-ISTRATI...
Topics
- Base Sequence
- Chromosome Deletion
- Chromosome Mapping
- DNA Probes
- DNA, Bacterial
- Dysentery, Bacillary
- Genes, Bacterial
- Humans
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Phenotype