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Article

Constructing and analyzing the fitness landscape of an experimental evolutionary process.

Chembiochem : a European journal of chemical biology - 22 Sept 2008

Reetz Manfred T, Sanchis Joaquin

Abstract excerpt

Iterative saturation mutagenesis (ISM) is a promising approach to more efficient directed evolution, especially for enhancing the enantioselectivity and/or thermostability of enzymes. This was demonstrated previously for an epoxide hydrolase (EH), after five sets of mutations led to a stepwise increase in enantioselectivity. This study utilizes these results to illuminate the nature of ISM, and identify the...

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Identifiers

PMID: 18712749
DOI: 10.1002/cbic.200800371

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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