Article
Surveying a local fitness landscape of a protein with epistatic sites for the study of directed evolution.
Biopolymers - 5 Jul 2002
Aita Takuyo, Hamamatsu Norio, Nomiya Yukiko, Uchiyama Hidefumi, Shibanaka Yasuhiko, Husimi Yuzuru
Abstract excerpt
We present a method for analysis of a fitness landscape of a biopolymer with significantly epistatic sites. The analysis is based on a quasi-additive fitness model. The fitness model is constructed with additive terms conducted by "site-fitness" and epistatic terms conducted by "pair-fitness," where the site-fitness is a fitness contribution from an independent residue and the pair-fitness is a fitness...
Topics
- Directed Molecular Evolution
- Half-Life
- Models, Molecular
- Mutation
- Prolyl Oligopeptidases
- Serine Endopeptidases
- Thermodynamics