Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Expression, purification and characterization of human PHD1 in Escherichia coli.

Journal of biochemistry - 1 Nov 2008

Li Xian Y, Takasaki Chikahisa, Satoh Yuhei, Kimura Shigenobu, Yasumoto Ken-Ichi, Sogawa Kazuhiro

Abstract excerpt

The hypoxia-inducible factors (HIFs) play a central role in oxygen homeostasis. HIF prolyl hydroxylases (PHDs) modify HIFalpha subunits and thereby target them for proteasomal degradation. Mammalian PHDs comprise three isozymes, PHD1, PHD2 and PHD3, and belong to the iron(II)-2-oxoglutarate-dependent dioxygenase family. We have expressed full-length human PHD1 in Escherichia coli, and purified it to apparent...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Dioxygenases
Share to Hypoxia-Inducible Factor 1, alpha Subunit

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Hypoxia-Inducible Factor-Proline Dioxygenases

Share to Isoenzymes

Share to Metals

Share to Procollagen-Proline Dioxygenase

Share to Recombinant Proteins

Source

Identifiers

PMID: 18710826
DOI: 10.1093/jb/mvn102

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Expression, purification and characterization of human PHD1 in Escherichia coli.PMID 18710826Read ↗

Biochemical characterization of human prolyl hydroxylase domain protein 2 variants associated with erythrocytosis.Semantic proximity Read ↗

Insights into the proline hydroxylase (PHD) family, molecular evolution and its impact on human health.Semantic proximity Read ↗

Characterization of an oxygen/redox-dependent degradation domain of hypoxia-inducible factor alpha (HIF-alpha) proteins.Semantic proximity Read ↗

Cloning and expression of recombinant human superoxide dismutase 1 (hSOD1) in Bacillus subtilis 1012Semantic proximity Read ↗

The PMS2 Subunit of Human MutLα Contains a Metal Ion Binding Domain of the Iron-Dependent Repressor Protein FamilySemantic proximity Read ↗

Expression and purification of full length mouse metal response element binding transcription factor-1 using Pichia pastoris.Semantic proximity Read ↗

Purification and mechanism of human aldehyde oxidase expressed in Escherichia coli.Semantic proximity Read ↗

Asparagine hydroxylation of the HIF transactivation domain a hypoxic switch.Semantic proximity Read ↗

PHD1 interacts with ATF4 and negatively regulates its transcriptional activity without prolyl hydroxylation.Semantic proximity Read ↗

Identification of the ubiquitin-protein ligase that recognizes oxidized IRP2.Semantic proximity Read ↗

An easy method for bacterial expression and purification of wild-type and mutant superoxide dismutase 1 (SOD1).Semantic proximity Read ↗

Depletion of intracellular ascorbate by the carcinogenic metals nickel and cobalt results in the induction of hypoxic stress.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.