Article
A sensitive detection method for MPLW515L or MPLW515K mutation in chronic myeloproliferative disorders with locked nucleic acid-modified probes and real-time polymerase chain reaction.
The Journal of molecular diagnostics : JMD - 1 Sept 2008
Pancrazzi Alessandro, Guglielmelli Paola, Ponziani Vanessa, Bergamaschi Gaetano, Bosi Alberto, Barosi Giovanni, Vannucchi Alessandro M
Abstract excerpt
Acquired mutations in the juxtamembrane region of MPL (W515K or W515L), the receptor for thrombopoietin, have been described in patients with primary myelofibrosis or essential thrombocythemia, which are chronic myeloproliferative disorders. We have developed a real-time polymerase chain reaction assay for the detection and quantification of MPL mutations that is based on locked nucleic acid fluorescent probes....
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