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Article

Probing the antigenicity of E. coli L-asparaginase by mutational analysis.

Molecular biotechnology - 1 May 2006

Jianhua Chen, Yujun Wei, Ruibo Jia, Min Wang, Wutong Wu

Abstract excerpt

A strategy, termed alanine-scanning mutagenesis, was used to identify the amino acid residues which are critical to the antigenicity of Escherichia coli L-asparaginase (L-ASP). Three continuous alkaline residues, 195RKH197, were mutated to Ala selectively. Four mutant recombinant L-ASPs were constructed and expressed in E. coli, and then purified. The purified mutants showed a single band by sodium dodecyl...

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PMID: 16691007
DOI: 10.1385/MB:33:1:57

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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