Article
Virus attenuation by genome-scale changes in codon pair bias.
Science (New York, N.Y.) - 27 Jun 2008
Coleman J Robert, Papamichail Dimitris, Skiena Steven, Futcher Bruce, Wimmer Eckard, Mueller Steffen
Abstract excerpt
As a result of the redundancy of the genetic code, adjacent pairs of amino acids can be encoded by as many as 36 different pairs of synonymous codons. A species-specific "codon pair bias" provides that some synonymous codon pairs are used more or less frequently than statistically predicted. We synthesized de novo large DNA molecules using hundreds of over-or underrepresented synonymous codon pairs to encode the...
Topics
- Algorithms
- Animals
- Antibodies, Viral
- Capsid Proteins
- Cloning, Molecular
- Codon
- Cytopathogenic Effect, Viral
- Genome, Viral
- HeLa Cells
- Hot Temperature
- Humans
- Mice
- Mice, Transgenic
- Mutation
- Poliomyelitis