Article
A purple acidophilic di-ferric DNA ligase from Ferroplasma.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jul 2008
Ferrer Manuel, Golyshina Olga V, Beloqui Ana, Böttger Lars H, Andreu José M, Polaina Julio, De Lacey Antonio L, Trautwein Alfred X, Timmis Kenneth N, Golyshin Peter N
Abstract excerpt
We describe here an extraordinary purple-colored DNA ligase, LigFa, from the acidophilic ferrous iron-oxidizing archaeon Ferroplasma acidiphilum, a di-ferric enzyme with an extremely low pH activity optimum. Unlike any other DNA ligase studied to date, LigFa contains two Fe(3+)-tyrosinate centers and lacks any requirement for either Mg(2+) or K(+) for activity. DNA ligases from closest phylogenetic and...
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