Conserved residues in domain Ia are required for the reaction of Escherichia coli DNA ligase with NAD+.

doi:10.1074/jbc.M111164200

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Article

Conserved residues in domain Ia are required for the reaction of Escherichia coli DNA ligase with NAD+.

The Journal of biological chemistry - 22 Mar 2002

Sriskanda Verl, Shuman Stewart

Abstract excerpt

NAD(+)-dependent DNA ligases are present in all bacteria and are essential for growth. Their unique substrate specificity compared with ATP-dependent human DNA ligases recommends the NAD(+) ligases as targets for the development of new broad-spectrum antibiotics. A plausible strategy for drug discovery is to identify the structural components of bacterial DNA ligase that interact with NAD(+) and then to isolate...

Topics

Alanine
Amino Acid Sequence
Aspartic Acid
DNA Ligases
Dose-Response Relationship, Drug
Escherichia coli
Gene Deletion
Histidine
Kinetics
Ligands
Models, Biological

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

NAD

Protein Binding

Protein Conformation

Protein Structure, Tertiary

Sequence Homology, Amino Acid

Source

Open in PubMed

Identifiers

PMID: 11781321
DOI: 10.1074/jbc.M111164200

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