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Article

Structure-based mutational study of an archaeal DNA ligase towards improvement of ligation activity.

Chembiochem : a European journal of chemical biology - 26 Nov 2012

Tanabe Maiko, Ishino Sonoko, Yohda Masafumi, Morikawa Kosuke, Ishino Yoshizumi, Nishida Hirokazu

Abstract excerpt

DNA ligases catalyze the joining of strand breaks in duplex DNA. The DNA ligase of Pyrococcus furiosus (PfuLig), which architecturally resembles the human DNA ligase I (hLigI), comprises an N-terminal DNA-binding domain, a middle adenylylation domain, and a C-terminal oligonucleotide-binding (OB)-fold domain. Here we addressed the C-terminal helix in the OB-fold domain of PfuLig by mutational analysis. The...

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Identifiers

PMID: 23132734
DOI: 10.1002/cbic.201200336

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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