Article
A suppressor of yeast spp81/ded1 mutations encodes a very similar putative ATP-dependent RNA helicase.
Molecular microbiology - 1 Apr 1991
Jamieson D J, Beggs J D
Abstract excerpt
The spp81/ded1 mutations were isolated as suppressors of a Saccharomyces cerevisiae pre-mRNA splicing mutation, prp8-1. The SPP81/DED1 gene encodes a putative ATP-dependent RNA helicase. While attempting to clone the wild-type SPP81/DED1 gene we isolated plasmids which were able to suppress the cold-sensitive growth defect of spp81 mutants. These plasmids encoded a gene (named DBP1) which mapped to chromosome XVI...
Topics
- Adenosine Triphosphate
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Cell Survival
- Cloning, Molecular
- DEAD-box RNA Helicases
- Genes, Fungal
- Genes, Suppressor
- Molecular Sequence Data
- Mutation