Motif III in superfamily 2 "helicases" helps convert the binding energy of ATP into a high-affinity RNA binding site in the yeast DEAD-box protein Ded1.

Abstract excerpt

Motif III in the putative helicases of superfamily 2 is highly conserved in both its sequence and its structural context. It typically consists of the sequence alcohol-alanine-alcohol (S/T-A-S/T). Historically, it was thought to link ATPase activity with a "helicase" strand displacement activity that disrupts RNA or DNA duplexes. DEAD-box proteins constitute the largest family of superfamily 2; they are...

Topics

• Adenosine Triphosphatases
• Adenosine Triphosphate
• Amino Acid Motifs
• Amino Acid Sequence
• Binding Sites
• Conserved Sequence
• DEAD-box RNA Helicases
• Genes, Fungal
• Kinetics
• Models, Molecular
• Molecular Sequence Data
• Mutation
• Phenotype