Article
Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm.
Scientific reports - 6 Jan 2016
El Khatib Mariam, Martins Alexandre, Bourgeois Dominique, Colletier Jacques-Philippe, Adam Virgile
Abstract excerpt
Phototransformable fluorescent proteins are central to several nanoscopy approaches. As yet however, there is no available variant allowing super-resolution imaging in cell compartments that maintain oxidative conditions. Here, we report the rational design of two reversibly switchable fluorescent proteins able to fold and photoswitch in the bacterial periplasm, rsFolder and rsFolder2. rsFolder was designed by...
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