Article
Disruption of the gene encoding the p34/31 polypeptides affects growth and development of Dictyostelium discoideum.
Molecular & general genetics : MGG - 1 Apr 1991
Bain G, Tsang A
Abstract excerpt
We have used homologous recombination to disrupt the gene which codes for p34 and p31, two polypeptides related to a cAMP-binding protein (CABP1) in Dictyostelium discoideum. By screening a total of 80 independent transformants by Southern blotting, four mutants have been isolated. Two of these mutants were analyzed in detail. Our results indicate that, while a null allele has not been obtained, both mutants...
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