Disruption of the gene encoding the p34/31 polypeptides affects growth and development of Dictyostelium discoideum.

doi:10.1007/BF00273587

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Article

Disruption of the gene encoding the p34/31 polypeptides affects growth and development of Dictyostelium discoideum.

Molecular & general genetics : MGG - 1 Apr 1991

Bain G, Tsang A

Abstract excerpt

We have used homologous recombination to disrupt the gene which codes for p34 and p31, two polypeptides related to a cAMP-binding protein (CABP1) in Dictyostelium discoideum. By screening a total of 80 independent transformants by Southern blotting, four mutants have been isolated. Two of these m...

Topics

Alleles
Blotting, Southern
Carrier Proteins
Cyclic AMP
Cyclic AMP Receptor Protein
DNA, Fungal
Dictyostelium
Genes, Fungal
Mutation
Phenotype
Recombination, Genetic
Restriction Mapping
Transformation, Genetic

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