Article
Transposon mutagenesis reveals novel loci affecting tolerance to salt stress and growth at low temperature.
Current genetics - 1 Aug 2001
de Jesus Ferreira M C, Bao X, Laizé V, Hohmann S
Abstract excerpt
Using transposon mutagenesis in the haploid Saccharomyces cerevisiae strain W303-1A we have identified genes required for growth in high salt medium, survival of a hypo-osmotic shock and growth at 15 degrees C. Screening 25,000 transposon insertions revealed a total of 61 insertions that caused salt-sensitivity; and those insertions affected 31 genes. Only 12 of those genes were previously known to be required...
Topics
- Base Sequence
- Cold Temperature
- DNA Transposable Elements
- Gene Deletion
- Genes, Fungal
- Mutagenesis, Insertional
- Mutation
- Osmotic Pressure
- Phenotype
- Saccharomyces cerevisiae
- Sodium Chloride