Article
A cystic fibrosis bronchial epithelial cell line: immortalization by adeno-12-SV40 infection.
American journal of respiratory cell and molecular biology - 1 Apr 1991
Zeitlin P L, Lu L, Rhim J, Cutting G, Stetten G, Kieffer K A, Craig R, Guggino W B
Abstract excerpt
An immortalized cell line was created from a primary culture of bronchial epithelia isolated from a patient with cystic fibrosis. The culture was transformed with a hybrid virus, adeno-12-SV40, which has been used successfully on a number of different human epithelial tissues. The transformed bronchial epithelial cells have the following characteristics. (1) Cyclic adenosine monophosphate (cAMP) is stimulated by...
Topics
- Antigens, Viral, Tumor
- Cell Line
- Cell Transformation, Viral
- Child
- Chloride Channels
- Chromosome Deletion
- Cyclic AMP
- Cystic Fibrosis
- DNA, Satellite
- Epithelium
- Humans