Article
Expression of normal and cystic fibrosis phenotypes by continuous airway epithelial cell lines.
The American journal of physiology - 1 Dec 1990
Jefferson D M, Valentich J D, Marini F C, Grubman S A, Iannuzzi M C, Dorkin H L, Li M, Klinger K W, Welsh M J
Abstract excerpt
Continuous epithelial cell lines from individuals with cystic fibrosis (CF) and normal controls are required to understand the genetic and cellular defects in CF. We used retroviruses to transduce SV40 large T antigen into nasal epithelial cells. Transformed continuous cell lines were isolated that expressed epithelial markers, cytokeratin, and tight junctions. Northern blot analysis shows that all of the cell...
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