Article
Oncogene-mediated propagation of tracheal epithelial cells from two cystic fibrosis fetuses with different mutations. Characterization of CFT-1 and CFT-2 cells in culture.
European journal of clinical investigation - 1 Mar 1993
Lemnaouar M, Chastre E, Paul A, Mergey M, Veissière D, Cherqui G, Barbry P, Simon-Bouy B, Fanen P, Gespach C
Abstract excerpt
Primary tracheal epithelial cells obtained from two fetuses with cystic fibrosis (CF) were successfully transfected with a plasmid vector recombined with the large T oncogene of SV40. The resulting tracheal cells were propagated in culture for up to 25 passages and retained the mutations of the CF genes carried by the two fetuses, one heterozygous for the S549N and N1303K substitutions (CFT-1 cells), and the...
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