Article
Oncogene-mediated propagation of tracheal epithelial cells from two cystic fibrosis fetuses with different mutations. Characterization of CFT-1 and CFT-2 cells in culture.
European journal of clinical investigation - 1 Mar 1993
Lemnaouar M, Chastre E, Paul A, Mergey M, Veissière D, Cherqui G, Barbry P, Simon-Bouy B, Fanen P, Gespach C
Abstract excerpt
Primary tracheal epithelial cells obtained from two fetuses with cystic fibrosis (CF) were successfully transfected with a plasmid vector recombined with the large T oncogene of SV40. The resulting tracheal cells were propagated in culture for up to 25 passages and retained the mutations of the C...
Topics
- Animals
- Cell Transformation, Neoplastic
- Cells, Cultured
- Cystic Fibrosis
- Cystic Fibrosis Transmembrane Conductance Regulator
- Epithelium
- Female
- Fetus
- Gene Expression
- Humans
- Membrane Proteins
- Mice
- Mice, Nude
- Mutation