Characterization of SV40 enhancer motifs involved in positive and negative regulation of the constitutive late promoter activity; effect of T-antigen.

Abstract excerpt

By analyzing the late promoter activity of a series of nonreplicative recombinants mutated within the different enhancer motifs of SV40 we identified both positive and negative regulatory elements. In the absence of T-antigen, the motifs Sph and/or octamer, and to a lesser extent the motifs GTI and P, account for the constitutive expression of the late promoter. The motif GTII overlaps elements that negatively...

Topics

• Antigens, Viral, Tumor
• Base Sequence
• Enhancer Elements, Genetic
• Gene Expression Regulation, Viral
• Molecular Sequence Data
• Mutation
• Plasmids
• Promoter Regions, Genetic
• Simian virus 40