Article
Cloning of the two essential yeast genes, PRP6 and PRP9, and their rapid mapping, disruption and partial sequencing using a linker insertion strategy.
Molecular & general genetics : MGG - 1 Feb 1991
Legrain P, Chapon C, Schwob E, Martin R, Rosbash M, Dujon B
Abstract excerpt
In the yeast Saccharomyces cerevisiae, some thermosensitive (ts) mutants have been shown to be impaired in pre-mRNA splicing (prp mutants). From a yeast genomic library, we have isolated plasmids that complement prp6 or prp9 ts mutations. These plasmids also complement the ts growth defect of add...
Topics
- Base Sequence
- Chromosome Mapping
- Chromosomes, Fungal
- Cloning, Molecular
- DNA Transposable Elements
- Genes, Fungal
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Open Reading Frames
- Plasmids
- RNA Splicing
- Restriction Mapping
- Saccharomyces cerevisiae