Article
Nuclear factor 1 activates the feline leukemia virus long terminal repeat but is posttranscriptionally down-regulated in leukemia cell lines.
Journal of virology - 1 Apr 1991
Plumb M, Fulton R, Breimer L, Stewart M, Willison K, Neil J C
Abstract excerpt
A recombinant feline leukemia virus (FeLV) proviral clone (T17T-22) with a long terminal repeat (LTR) which differs from prototype FeLV by a point mutation within a conserved nuclear factor 1 (NF1)-binding motif in the LTR enhancer domain was found to be poorly expressed after DNA transfection. The NF1 point mutation reduced in vitro protein binding as assessed by gel shift analysis and reduced promoter activity...
Topics
- Animals
- Base Sequence
- CCAAT-Enhancer-Binding Proteins
- Cats
- DNA, Viral
- DNA-Binding Proteins
- Down-Regulation
- Enhancer Elements, Genetic
- Gene Expression Regulation
- Leukemia Virus, Feline