Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

An Asp79Asn mutation of the alpha2A-adrenoceptor interferes equally with agonist activation of individual Gialpha-family G protein subtypes.

FEBS letters - 3 Dec 1999

Ward R J, Milligan G

Abstract excerpt

The quantitative effects of an Asp79Asn mutation in the porcine alpha2A-adrenoceptor on adrenaline-mediated stimulation of the alpha subunit of individual members of the Gi family of G proteins were assessed by measuring GTP turnover number for fusion proteins between the wild type or mutated receptor and pertussis toxin-resistant forms of each of Gi1, Gi2 and Gi3. In each case the receptor mutation limited...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Asparagine
Share to Aspartic Acid
Share to Epinephrine

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to GTP-Binding Protein alpha Subunits, Gi-Go

Share to GTP-Binding Proteins

Share to GTP Phosphohydrolases

Share to Heterotrimeric GTP-Binding Proteins

Share to Receptors, Adrenergic, alpha-2

Source

Identifiers

PMID: 10622745
DOI: 10.1016/s0014-5793(99)01581-1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

An Asp79Asn mutation of the alpha2A-adrenoceptor interferes equally with agonist activation of individual Gialpha-family G protein subtypes.PMID 10622745Read ↗

Interactions of the alpha2A-adrenoceptor with multiple Gi-family G-proteins: studies with pertussis toxin-resistant G-protein mutants.Semantic proximity Read ↗

Coupling of a mutated form of the human beta 2-adrenergic receptor to Gi and Gs. Requirement for multiple cytoplasmic domains in the coupling process.Semantic proximity Read ↗

Purification and characterization of the G203T mutant alpha i-2 subunit of GTP-binding protein expressed in baculovirus-infected Sf9 cells.Semantic proximity Read ↗

Closely related G-protein-coupled receptors use multiple and distinct domains on G-protein alpha-subunits for selective coupling.Semantic proximity Read ↗

Measurement of agonist-dependent and -independent signal initiation of alpha(1b)-adrenoceptor mutants by direct analysis of guanine nucleotide exchange on the G protein galpha(11).Semantic proximity Read ↗

Genetic and structural analysis of G protein alpha subunit regulatory domains.Semantic proximity Read ↗

Reciprocal mutations of highly conserved residues in transmembrane helices 2 and 7 of the alpha(2A)-adrenoceptor restore agonist activation of G(i1)alpha.Semantic proximity Read ↗

Direct Coupling of a Seven-Transmembrane-Span Receptor to a Gαi G-Protein Regulatory Motif Complex.Semantic proximity Read ↗

Functional domains of the mouse beta3-adrenoceptor associated with differential G protein coupling.Semantic proximity Read ↗

A mutation of the beta 2-adrenergic receptor impairs agonist activation of adenylyl cyclase without affecting high affinity agonist binding. Distinct molecular determinants of the receptor are involved in physical coupling to and functional activation of Gs.Semantic proximity Read ↗

Second site suppressor mutations of a GTPase-deficient G-protein alpha-subunit. Selective inhibition of Gbeta gamma-mediated signaling.Semantic proximity Read ↗

A Gsalpha mutant designed to inhibit receptor signaling through Gs.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.