Article
Mutations that alter the charge of type I regulatory subunit and modify activation properties of cyclic AMP-dependent protein kinase from S49 mouse lymphoma cells.
The Journal of biological chemistry - 25 Feb 1991
Steinberg R A, Gorman K B, Ogreid D, Døskeland S O, Weber I T
Abstract excerpt
Mutations in regulatory (R) subunit of cAMP-dependent protein kinase were analyzed from cAMP-resistant mutants of S49 mouse lymphoma cells by direct sequencing of amplified regions of mutant R subunit cDNAs. Eight distinct single base-change lesions were identified in 24 independent mutants that were hemizygous for expression of mutant R subunits with altered protein charge. CG----TA transitions predominated, but...
Topics
- Animals
- Base Sequence
- Binding Sites
- Cyclic AMP
- DNA
- Enzyme Activation
- Lymphoma
- Mice
- Models, Molecular
- Molecular Sequence Data
- Mutation