Article
Random mutagenesis of G protein alpha subunit G(o)alpha. Mutations altering nucleotide binding.
The Journal of biological chemistry - 15 Oct 1993
Slepak V Z, Quick M W, Aragay A M, Davidson N, Lester H A, Simon M I
Abstract excerpt
Nucleotide binding properties of the G protein alpha subunit G(o)alpha were probed by mutational analysis in recombinant Escherichia coli. Thousands of random mutations generated by polymerase chain reaction were screened by in situ [35S]GTP gamma S (guanosine 5'-(3-O-thio)-triphosphate) binding...
Topics
- Animals
- Binding Sites
- DNA Mutational Analysis
- Escherichia coli
- GTP-Binding Proteins
- Genes, Dominant
- Guanosine 5'-O-(3-Thiotriphosphate)
- Mutagenesis
- Oncogene Protein p21(ras)
- Phenotype
- Receptors, Thyrotropin-Releasing Hormone
- Recombinant Proteins
- Sequence Homology, Nucleic Acid
- Xenopus laevis