Article
Yeast cAMP-dependent protein kinase regulatory subunit mutations display a variety of phenotypes.
The Journal of biological chemistry - 15 Jul 1990
Cannon J F, Gitan R, Tatchell K
Abstract excerpt
Ten spontaneous and four in vitro constructed mutations in the gene encoding the regulatory subunit of cAMP-dependent protein kinase of Saccharomyces cerevisiae display very different phenotypes. The DNA nucleotide sequence of each spontaneous mutation was determined. Mutations were found in both the cAMP-binding domains and proximal to the cAMP-dependent protein kinase phosphorylation site. The latter mutations...
Topics
- Affinity Labels
- Amino Acid Sequence
- Azides
- Base Sequence
- Cyclic AMP
- Genes, Fungal
- Glycogen
- Kinetics
- Macromolecular Substances
- Models, Molecular
- Mutation
- Phenotype