Article
Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates.
Molecular cell - 9 May 2008
Li Fuyang, Dong Junchao, Pan Xuewen, Oum Ji-Hyun, Boeke Jef D, Lee Sang Eun
Abstract excerpt
Elimination of a double-strand break (DSB) flanked by direct repeat sequences is mediated by single-strand annealing (SSA), which relies on a distinct set of gene products involving recombination, mismatch repair, and nucleotide excision repair. Here, we screened for yeast mutants defective in SSA with a plasmid-based SSA assay coupled to a barcode microarray readout. The screen identified Yal027Wp/Saw1...
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