Influence of highly curved DNA segments on in vivo topology of plasmids.

doi:10.1023/a:1007017121313

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Article

Influence of highly curved DNA segments on in vivo topology of plasmids.

Molecular biology reports - 1 Dec 1999

Ohyama T, Miyano M, Sakuma S

Abstract excerpt

Recombinant plasmids carrying a highly curved DNA structure are sometimes unstable in Escherichia coli. In order to know the underlying mechanism, several plasmids carrying one or two highly bent DNA segment(s) from the human adenovirus type 2 (Ad2) enhancer and/or origin region of phage lambda replication were systematically constructed and propagated in E. coli. The highly bent DNA segments disturbed the action...

Topics

Adenoviruses, Human
Bacteriophage lambda
Base Sequence
DNA Topoisomerases, Type I
DNA, Viral
Electrophoresis, Gel, Two-Dimensional
Enhancer Elements, Genetic
Escherichia coli
Gene Deletion
Humans
Mutation
Nucleic Acid Conformation
Plasmids
Replication Origin
Sequence Analysis, DNA

Source

Open in PubMed

Identifiers

PMID: 10634510
DOI: 10.1023/a:1007017121313

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