Article
Hyper-negative template DNA supercoiling during transcription of the tetracycline-resistance gene in topA mutants is largely constrained in vivo.
Nucleic acids research - 1 Aug 1996
Albert A C, Spirito F, Figueroa-Bossi N, Bossi L, Rahmouni A R
Abstract excerpt
The excess linking deficit of plasmid DNA from topoisomerase I-defective bacteria (topA mutants) results mainly from transcription and is commonly ascribed to unbalanced relaxation of transcription-induced twin-supercoiled domains. This defect is aggravated in genes for membrane-binding proteins...
Topics
- Chloramphenicol O-Acetyltransferase
- DNA Topoisomerases, Type I
- DNA, Superhelical
- Genes, Bacterial
- Mutation
- Nucleic Acid Conformation
- Plasmids
- Protein Biosynthesis
- Repressor Proteins
- Tetracycline Resistance
- Transcription, Genetic