Article
A second-site revertant of a defective herpes simplex virus ICP4 protein with restored regulatory activities and impaired DNA-binding properties.
Journal of virology - 1 Feb 1991
Shepard A A, DeLuca N A
Abstract excerpt
A mutant of herpes simplex virus type 1, vi12, encodes a DNA-binding- and transactivation-deficient ICP4 polypeptide. Because of the mutation, the vi12 virus does not grow on Vero cells but must be propagated on cells that express complementing levels of wild-type ICP4 (E5 cells). A pseudoreverta...
Topics
- Amino Acid Sequence
- Animals
- Blotting, Southern
- Cloning, Molecular
- DNA, Viral
- DNA-Binding Proteins
- Defective Viruses
- Genotype
- Immediate-Early Proteins
- Molecular Sequence Data
- Plasmids
- Restriction Mapping
- Simplexvirus
- Transcription, Genetic
- Transcriptional Activation
- Vero Cells
- Viral Regulatory and Accessory Proteins