The conserved carboxyl-terminal half of herpes simplex virus type 1 regulatory protein ICP27 is dispensable for viral growth in the presence of compensatory mutations.

Abstract excerpt

ICP27 is an essential herpes simplex virus type 1 (HSV-1) immediate-early protein that regulates viral gene expression by poorly characterized mechanisms. Previous data suggest that its carboxyl (C)-terminal portion is absolutely required for productive viral infection. In this study, we isolated M16R, a second-site revertant of a viral ICP27 C-terminal mutant. M16R harbors an intragenic reversion, as...

Topics

• Alleles
• Amino Acid Sequence
• Animals
• Chlorocebus aethiops
• Frameshift Mutation
• Genetic Complementation Test
• Herpesvirus 1, Human
• Immediate-Early Proteins
• Molecular Sequence Data
• Vero Cells
• Viral Plaque Assay
• Virus Replication