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Article

Mutations in DNA gyrase result in novobiocin resistance in halophilic archaebacteria.

Journal of bacteriology - 1 Jan 1991

Holmes M L, Dyall-Smith M L

Abstract excerpt

We have developed a cloning vector for use in halophilic archaebacteria which has a novobiocin resistance determinant as a selectable marker. The resistance determinant, which was derived from the genome of a resistant mutant strain, was mapped to a site within a 6.7-kb DNA clone by using a recombination assay and was sequenced. An open reading frame of 1.920 nucleotides (640 amino acids) was identified, with the...

Topics

Amino Acid Sequence
Archaea
Bacillus subtilis
Base Sequence
DNA Topoisomerases, Type II
Drug Resistance, Microbial
Genes, Bacterial
Molecular Sequence Data
Mutation
Novobiocin
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA, Messenger

Restriction Mapping

Sequence Homology, Nucleic Acid

Source

Open in PubMed Open full text

Identifiers

PMID: 1846146
DOI: 10.1128/jb.173.2.642-648.1991
PMCID: PMC207055

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1846146-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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