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Article

Homologous gene knockout in the archaeon Halobacterium salinarum with ura3 as a counterselectable marker.

Molecular microbiology - 1 Feb 2000

Peck R F, DasSarma S, Krebs M P

Abstract excerpt

To facilitate the functional genomic analysis of an archaeon, we have developed a homologous gene replacement strategy for Halobacterium salinarum based on ura3, which encodes the pyrimidine biosynthetic enzyme orotidine-5'-monophosphate decarboxylase. H. salinarum was shown to be sensitive to 5-fluoroorotic acid (5-FOA), which can select for mutations in ura3. A spontaneous 5-FOA-resistant mutant was found to...

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PMID: 10672188
DOI: 10.1046/j.1365-2958.2000.01739.x

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