Article
Construction and characterization of recA mutant strains of Corynebacterium glutamicum and Brevibacterium lactofermentum.
Applied microbiology and biotechnology - 1 Dec 1994
Fitzpatrick R, O'Donohue M, Joy J, Heery D M, Dunican L K
Abstract excerpt
An internal fragment of the Corynebacterium glutamicum recA gene was amplified by the polymerase chain reaction (PCR) using degenerate primers corresponding to two short sequences that are well conserved in procaryotic RecA proteins. The deduced amino acid sequence of the amplified fragment shared significant homology with RecA sequences from other bacteria including the "invariant" and functionally conserved...
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