Article
New antibiotic resistance cassettes suitable for genetic studies in Borrelia burgdorferi.
Journal of molecular microbiology and biotechnology - 1 Jan 2003
Elias Abdallah F, Bono James L, Kupko John J, Stewart Philip E, Krum Jonathan G, Rosa Patricia A
Abstract excerpt
In this report we describe two distinct approaches to develop new antibiotic resistance cassettes that allow for efficient selection of Borrelia burgdorferi transformants. The first approach utilizes fusions of borrelial flagellar promoters to antibiotic resistance markers from other bacteria. The AACC1 gene, which encodes a gentamicin acetyltransferase, conferred a high level of gentamicin resistance in B....
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