Article
Determination of lamivudine-resistant variants of hepatitis B virus by denaturing gradient gel electrophoresis: a novel approach to monitoring drug resistance.
Medical science monitor : international medical journal of experimental and clinical research - 1 May 2008
Bielawski Krzysztof Piotr, Al-Soud Waleed Abu, Stalke Piotr, Charmuszko Urszula, Wadstrom Torkel
Abstract excerpt
BACKGROUND: A DGGE-based assay for hepatitis B virus (HBV) drug-resistance monitoring was designed and checked for feasibility. It detects mutations within the YMDD motif related to lamivudine resistance. MATERIAL/METHODS: The YMDD motif of HBV polymerase was amplified by the set of primers desig...
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