Article
GSTT1 and GSTM1 gene copy number analysis in paraffin-embedded tissue using quantitative real-time PCR.
Analytical biochemistry - 15 Jul 2008
Bediaga Naiara G, Alfonso-Sánchez Miguel A, de Renobales Mertxe, Rocandio Ana M, Arroyo Marta, de Pancorbo Marian M
Abstract excerpt
GSTT1 and GSTM1 genes possess an inherited deletion associated with a lack of enzyme activity. The heterozygous condition of this deletion is difficult to determine in low-quality DNA with existing PCR protocols. We designed and validated a multiplex real-time PCR assay by adapting the DeltaDeltaCt relative quantification method for the analysis of GSTT1 and GSTM1 markers to accurately differentiate the three...
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