Article
High-throughput genotyping method for glutathione S-transferase T1 and M1 gene deletions using TaqMan probes.
Research communications in molecular pathology and pharmacology - 1 Jan 1999
Shi M M, Myrand S P, Bleavins M R, de la Iglesia F A
Abstract excerpt
A high-throughput genotyping method has been developed to detect gene deletion polymorphisms of glutathione-S-transferase theta and mu (GSTT1 and GSTM1). This method utilizes the 5'-nuclease activity of Taq polymerase in conjunction with fluorogenic TaqMan probes. In contrast to traditional allelic discrimination genotyping to detect single nucleotide polymorphisms, the current assay has been designed to detect...
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