Article
A novel NIH/3T3 duplex feeder system to engineer corneal epithelial sheets with enhanced cytokeratin 15-positive progenitor populations.
Tissue engineering. Part A - 1 Jul 2008
Miyashita Hideyuki, Shimmura Shigeto, Higa Kazunari, Yoshida Satoru, Kawakita Tetsuya, Shimazaki Jun, Tsubota Kazuo
Abstract excerpt
Corneal epithelial cell sheets co-cultivated with feeder cells are used to reconstruct the ocular surface in stem cell-depleted eyes. The present study was conducted to investigate the optimal method of using feeder cells in the interest of preserving progenitor cells in cultivated sheets. We compared the phenotype and secondary colony forming efficiency (CFE) of cell sheets that were engineered using 3T3 feeder...
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