Article
Kinetics of expansion of human limbal epithelial progenitor cells in primary culture of explants without feeders.
PloS one - 1 Jan 2013
Ghoubay-Benallaoua Djida, Sandali Otman, Goldschmidt Pablo, Borderie Vincent
Abstract excerpt
The aims of this study were to determine whether human limbal explant cultures without feeder cells result in expansion of epithelial progenitors and to estimate the optimal expansion time for progenitor cells. Limbal explants from ten human corneas were cultured for 7, 9, 11, 14, 18, and 21 days. Limbal explants from two corneas were enzymatically dissociated or directly cultured for 14 days. Progenitor cells...
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