Article
The protein truncation test in mutation detection and molecular diagnosis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2007
Hauss Oliver, Müller Oliver
Abstract excerpt
The protein truncation test (PTT) is a simple and fast method to screen for biologically relevant gene mutations. The method is based on the size analysis of products resulting from in vitro transcription and translation. Proteins of lower mass than the expected full-length protein represent translation products derived from truncating frame shift or stop mutations in the analyzed gene. Because of the low...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.