Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Screening for unknown mutations by a bioluminescent protein truncation test with homogeneous detection.

Journal of the American Chemical Society - 14 Apr 2010

Petrakis Evangelos C, Trantakis Ioannis A, Kalogianni Despina P, Christopoulos Theodore K

Abstract excerpt

The protein truncation test (PTT) is important in screening for unknown mutations that cause premature termination of mRNA translation. PTT involves amplification of the interrogated sequence, in vitro transcription/translation, separation of the generated polypeptides, and detection. In this article, we report a bioluminescent protein truncation test, in which the detection of the nascent protein is performed...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA
Share to APC gene
Share to Luminescent Measurements

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutation

Share to Sensitivity and Specificity

Share to Terminator Regions, Genetic

Share to Transcription, Genetic

Source

Identifiers

PMID: 20232860
DOI: 10.1021/ja909200p

Genes

APC

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Screening for unknown mutations by a bioluminescent protein truncation test with homogeneous detection.PMID 20232860Read ↗

The protein truncation test: A review.Semantic proximity Read ↗

Protein truncation test.Semantic proximity Read ↗

The protein truncation test in mutation detection and molecular diagnosis.Semantic proximity Read ↗

Protein truncation test (PTT) for rapid detection of translation-terminating mutationsSemantic proximity Read ↗

Development of a Premature Stop Codon-detection method based on a bacterial two-hybrid system.Semantic proximity Read ↗

A complete protein truncation test for BRCA1 and BRCA2.Semantic proximity Read ↗

Introduction of a myc reporter tag to improve the quality of mutation detection using the protein truncation test.Semantic proximity Read ↗

Application of the protein truncation test (PTT) for the detection of tuberculosis sclerosis complex type 1 and 2 (TSC1 and TSC2) mutations.Semantic proximity Read ↗

Direct analysis for familial adenomatous polyposis mutations.Semantic proximity Read ↗

Aequorin variants with improved bioluminescence properties.Semantic proximity Read ↗

Requirement of the C-terminal proline residue for stability of the Ca(2+)-activated photoprotein aequorin.Semantic proximity Read ↗

Enhanced sensitivity of a modified protein truncation test for exons 1-14 of the adenomatous polyposis coli gene.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.