Article
Design and application of noncontinuously binding probes used for haplotyping and genotyping.
Clinical chemistry - 1 Jun 2008
Pont-Kingdon Genevieve, Margraf Rebecca L, Sumner Kelli, Millson Alison, Lyon Elaine, Schütz Ekkehard
Abstract excerpt
BACKGROUND: Many methods for genotyping use melting temperature (Tm) of sequence-specific probes. Usually the probes hybridize to a continuous stretch of DNA that contains the variant(s). In contrast, hybridization of noncontinuous probes to a template can form bulges. This report generates guidelines for the design of noncontinuous probes. METHODS: We used software to predict hybridization structures and Tms...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.