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Article

SNP genotyping through the melting analysis of unlabelled oligonucleotide applied on dilute PCR amplicon.

Journal of biotechnology - 20 Jul 2011

Jeong Sangkyun, Yu Hyunjoo, Lee Yeongshin, Kim Jong Yeol

Abstract excerpt

Adaptation of DNA melting analysis for polymorphic single nucleotides (SNPs) genotyping using an unlabeled oligonucleotide probe for polymorphic DNAs under the presence of fluorescent DNA binding dye necessitates a reaction condition where the probe efficiently associates with a target strand that is PCR amplified. We present experimental evidence that application of an unlabeled probe to a dilute PCR amplicon...

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Identifiers

PMID: 21679732
DOI: 10.1016/j.jbiotec.2011.05.017

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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